HPLC Analysis of Caffeine Essay Example for Free

HPLC Analysis of Caffeine look forThe main objective of this lab is to gain experience in using and reading results from the HPLC machine. 2) The first mis call is to inject a series of caffeine standards into the machine in order to get results/values of raising height and area. 3) The next step is to investigate the effects of a series of HPLC parameters on Retention time, bill height and peak area. 4) To use above data from peak height and area to construct two standard curves vs concentration. Introduction Caffeine is a common organic molecule found in many an(prenominal) beverages such as coffee, tea, and cola.It is a stimulant to the central nervous system. Which is why the majority of the population use it in one form or another to help stay alert. In general hatful drink caffeinated drinks like the ones mentioned above in order to obtain their fix. However with many fresh studies in the area caffeine tablets are be flood tide a very popular way for athletes in lo ts of different sports to stay alert even when fatigue is setting in. HPLC stands for High Performance smooth Chromatography but is too referred to as High Pressure Liquid Chromatography.It is used for separating multifariousnesss either to analyse the change or to separate a required product from others in a reaction mixture. It can also be used to find the relative amounts of different fixingss in a mixture. HPLC works along the analogous lines as paper chromatography. In paper chromatography a liquid (mobile phase) moves past a solid (stationary phase). In paper chromatography the stationary phase consists of water molecules bound to the cellulose in the paper, the mobile phase carries different components of the mixture along with it.How fast each one moves depends on its relative affinity for the mobile and stationary phases. In HPLC the stationary phase is a solid packed into a newspaper column and the liquid is forced by the column by high pressure pumps. The pumps fo rce the mixed solvents through the column the solvent acclivitous from the column carries the separated components of the mixture and is passed into the detector where a beam of ultraviolet light shines through it. in that respect are many different types of detector depending on what you areanalysing. This light is at a wavelength that is oblivious by all the components to be separated. When the detector reading drops the component that is absorbing the UV light is coming out of the column and passing through the detector. The time it takes for each component to come off the column is called its retention time and can be used to help identify it. The more polar component comes off the column first followed by the less polar. Materials As per laboratory manual.